variant toll-like receptor4 (asp299gly and thr399ile alleles) and toll-like receptor2 (arg753gln and arg677trp alleles) in colorectal cancer
نویسندگان
چکیده
the innate immune system recognizes the presence of bacterial products through the expression of a family of membrane receptors known as toll-like receptors (tlrs). polymorphisms in tlrs have been shown to be associated with increased susceptibility to diseases such as inflammatory bowel disease. the aim of this study was to determine whether there was a correlation between polymorphisms of tlr4 (asp299gly; thr399ile) and tlr2 (arg677trp; arg753gln) genes and risk of colorectal cancer. dna from 60 colorectal carcinoma patients from 3 major races in malaysia (22 malays, 20 chinese and 18 indians) and blood from 50 apparently healthy individuals were evaluated. control group were matched to study group by race and age. the polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). genotyping results showed two out of sixty tumor specimens (3.3%) harbored both variant tlr4 asp299gly and thr399ile alleles. in contrast, dna isolated from blood cells of 50 apparently healthy individuals harbored wild type tlr4. in the case of tlr2 arg753gln genotyping, all of the fifty normal and 60 tumors were of the wild type genotype. tlr2 arg677trp genotyping showed a heterozygous pattern in all samples. however, this may not be a true polymorphism of the tlr2 gene as it is likely due to a variation of a duplicated (pseudogene) region. there was only a low incidence (2/60; 3.3%) of tlr4 polymorphism at the asp299gly and thr399ile alleles in colorectal cancer patients. all normal and tumor samples harbored the wild type tlr2 arg753 allele. our study suggests that variant tlr4 (asp299gly and thr399ile alleles) as well as tlr2 (arg753gln allele) are not associated with risk of colorectal cancer.
منابع مشابه
Variant Toll-like receptor4 (Asp299Gly and Thr399Ile alleles) and Toll-like receptor2 (Arg753Gln and Arg677Trp alleles) in colorectal cancer.
The innate immune system recognizes the presence of bacterial products through the expression of a family of membrane receptors known as Toll-like receptors (TLRs). Polymorphisms in TLRs have been shown to be associated with increased susceptibility to diseases such as inflammatory bowel disease. The aim of this study was to determine whether there was a correlation between polymorphisms of TLR...
متن کاملبررسی چند شکلی ژنهای toll like receptor2 arg753gln و toll like receptor4 asp299gly دربیماران مبتلا به آسم ایرانی در مقایسه با گروه کنترل
مقدمه: آسم بیماری التهابی مجاری تنفسی است که عوامل ژنتیکی و محیطی مختلفی در آن نقش دارند.بیماری آسم را از جنبه های ایمونولوژیک می توان مورد بررسی قرار داد. ژن های مختلفی از سیستم ایمنی در ایجاد و تشدید آسم دخیل هستند. از جمله این ژن ها در سیستم ایمنی خانواده tlrs می باشد.از انجا که ایجاد چندشکلی ژنی در ژنها باعث تغییر در نحوه پاسخ سیستم ایمنی می گردد در این مطالعه به بررسی چند شکلی ژنی در خانوا...
15 صفحه اولToll-like receptor 4 single-nucleotide polymorphisms Asp299Gly and Thr399Ile in ovarian cancers
Toll-like receptor (TLR4) 4 is present in numerous cell types and serves as the first point of defense in the innate immune system. Single-nucleotide polymorphisms (SNPs) are present in a number TLR genes and have been associated with various infection and inflammation disorders. Asp299Gly and Thr399Ile, TLR4 SNPs, are associated with tumor progression. In the present study, cases of ovarian ca...
متن کاملToll-like receptor 4 Asp299Gly and Thr399Ile polymorphisms: New data and a meta-analysis
BACKGROUND The pathogenesis of inflammatory bowel disease (IBD) involves interactions between the host genetic susceptibility, intestinal microflora and mucosal immune responses through the pattern recognition receptor. Polymorphisms in toll-like receptor 4 (TLR4) induce an aberrant immune response to indigenous intestinal flora, which might favor IBD development. In this study, we aimed to det...
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متن کاملAbsence of the toll-like receptor 4 gene polymorphisms Asp299Gly and Thr399Ile in Singaporean Chinese
Toll-like receptor 4 (TLR4) is the transuding subunit of lipopolysaccharide receptor and an important intracellular signal pathway of the innate immune response. Published data about the relationship between TLR4 mutations and atopy/asthma are not consistent. This study was performed to detect two commonly reported Asp299Gly and Thr399Ile polymorphisms in TLR4 gene in Singaporean Chinese and th...
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عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۰، شماره ۲، صفحات ۹۱-۹۹
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